This article discusses on the interaction between plasma proteins with other proteins. The research was done using two brands of PVC tubing which were untreated medical grade, phosphorylcholine-coated PVC tubing, triblock-copolymer (polycaprolactone-polydimethylsiloxane-polycaprolactone)-treated PVC tubing and poly-2-methoxyethylacrylate (PMEA). The coated tubing were exposed to the human plasma for 60 minutes. Then, a broad spectrum of plasma protein was found on all tubing. The ratio was lower for the adsorbed albumin to total protein that the parallel ratio of plasma while 1 and 2 globulins are over-represented in the protein spectrum.
The protein layer was at an average thickness of about 23mm. Therefore, it highlights the point that plasma protein adsorption exists on uncoated and protein-repellent tubing and therefore can set off a systemic inflammatory reaction. Among the limitations highlighted were that the results may have not completely able to reflect the clinical situation and that although plasma had a higher adsorption rate of protein than blood, it was used and therefore may be a limitation as well. Another limitation highlighted was that the study had not examined the conformational changes in the structure of protein.
Development and validation of an HPLC method to quantify camptothecin in polymeric nanocapsule suspensions. By: Granada, Andrea,Murakami, Fabio S. ,Sartori, Tatiane,Lemos-Senna, Elenara,Silva, Marcos A. S. (2008) The article highlights that a method was developed and therefore validated to quantify the camptothecin (CPT) in polymeric nanocapsule suspensions. To determine the total CPT, it was performed at pH 2. 8 and was validated by evaluating the efficiency of the entrapment of the CPT into the nanocapsules.
Upon several attempts, the peak results were achieved when the volume ratio of methanol and 10 mM phosphoric acid buffer was 60 + 40 (v/v). Apart from that, the sturdiness of the method was examined. According to the results, the variations were slight and therefore imposed no major effect on the data and therefore prove that it is suitable to be used for other laboratories. Furthermore, the Table 4 results as highlighted in the article, proves that the method was successful in determining the CPT content and in exploring the drug entrapment efficient in polymeric nanocapsule suspensions.
Therefore, as a conclusion, this proved that the methods used were accurate and therefore could be used for other similar research. Development and application of molecularly imprinted polymers as solid-phase sorbents for erythromycin extraction (Song, S. , Wu, A. , Shi, X. , Li, R. , Lin, Z. , Zhang, D, 2008) This articles highlights that based on the analysis done, the MIPs had more convergent porous structures than the non imprinted polymers. Besides that, the binding experiments demonstrated that the MIPs binding sites were heterogeneous with two dissociation.
Apart from that, the MIPs performance was viewed as being a solid-phase extraction (SPE) sorbents were also evaluated. The selectivity analysis then demonstrated that the MIPs could identify ERY with cross-reactivity at a moderate level for other macrolides. Therefore, upon investigation, it was highlighted that the viability of using the MIPs obtained as specific SPE sorbents for ERY extraction is viable in real samples. As a conclusion, there were several methods and results highlighted from the various articles above on the polymers and its affect on the body.
However, in some cases, there is still a need for further research and in some cases; it can already be used for practical use.
References:
Gooptu, B & Lomas, D. A (2008) Polymers and inflammation: disease mechanisms of the serpinopathies. The Rockefeller University Press, Vol 205, 1-7 Granada, Andrea,Murakami, Fabio S. ,Sartori, Tatiane,Lemos-Senna, Elenara,Silva, Marcos A. S. (2008) Development and validation of an HPLC method to quantify camptothecin in polymeric nanocapsule suspensions. Journal of AOAC, Vol 91, No 3, 551-56
Somer, F. D. , Landschoot, A. V. , Nooten, G. V. , Delanghe, J (2008) Interaction of plasma proteins with commercial protein repellent polyvinyl chloride (PVC): a word of caution. SAGE publications, 1-8 Song, S. , Wu, A. , Shi, X. , Li, R. , Lin, Z. , Zhang, D (2008) Development and application of molecularly imprinted polymers as solid-phase sorbents for erythromycin extraction. Anal Bioanal Chem,390: 2141-2150. Ward, R. S (2008) New Horizons for Biomedical Polymers. Medical Device Technology, 1-6